Fig. 4

Adhesion of pre-cultured BMMs to extracellular matrix components and expression of integrin CD29. Adhesion to extracellular matrix components collagen type I (a, b), fibronectin (c, d), and laminin (e, f) was measured 36 h after seeding, and cultured BMMs from controls and from CIA animals (a, c, e) 20 and 40 days post immunization were compared. Data are shown as percentage of BMMs from control animals set to 100 %. Influence of stimulation with ACh, VIP, and NA on extracellular matrix adhesion of BMMs from control and CIA animals is shown as percentage to non-stimulated macrophages (100 % = solid line). Results for collagen type I are shown under (b), for fibronectin under (d), and respective results for laminin are shown under (f). *P < 0.05 neurotransmitter stimulation versus non-stimulated cells; ^## P < 0.01 control cells versus CIA cells. g, h BMMs were analyzed by flow cytometry after 2 days of pre-culture. BMMs were positive for CD29 (integrin β1, g). h compares expression levels of CD29 on BMMs from control and arthritic animals 20 and 40 days post immunization. N = 6 for control and CIA at each time point. Boxplots show box with median and interquartile range and whiskers from minimum to maximum. ACh acetylcholine, BMM bone marrow-derived macrophage, CIA collagen II-induced arthritis, NA noradrenaline, VIP vasoactive intestinal peptide