Fig. 1

Serum nucleases degrade chromatin in primary and secondary necrotic cells. Annexin V and Via-Probe was used to define live (a), apoptotic (b) primary necrotic (Prim-Nec) (c) and secondary necrotic (Sec-Nec) (d) cell states in Jurkat T-cells. The degradation of chromatin in live (e), apoptotic (f) primary (g) and secondary necrotic (h) cellular DNA content was determined after incubating cells with normal human serum (NHS) or heat-inactivated normal human serum (Hi-NHS). Cells with subnormal DNA content as indicated by the vertical gate were quantified for different serum concentrations and displayed as percentage of the total cell population (i-l). a-h Representative histograms of three independent experiments using 3 % serum in e-h. i-l Mean ± SD of e-h at varying serum concentrations. Significance of difference was calculated using two-way analysis of variance with the Bonferroni post hoc test; ***p <0.001