Fig. 3
From: Expression of Semaphorin 4A and its potential role in rheumatoid arthritis
Effects of lipopolysaccharide (LPS) on the expression and secretion of semaphorin 4A (Sema4A). qRT-PCR was applied to detect the expression of Sema4A after stimulation by LPS at various concentrations (a) or periods (b) in synovial fibroblasts of rheumatoid arthritis RASFs, respectively. c Sema4A protein was assayed by western blot after LPS treatment in RASFs at indicated time points. d Secretion of Sema4A in the supernatant of RASFs was detected by ELISA after LPS stimulation. *P <0.05 and **P <0.01 (statistically significant differences) GAPDH glyceraldehyde-3-phosphate dehydrogenase