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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Expression of Semaphorin 4A and its potential role in rheumatoid arthritis

Fig. 5

Semaphorin 4A (Sema4A) promotes IL-6 production in an NF-κB dependent way. a Synovial fibroblasts of rheumatoid arthritis (RASFs) were treated with recombinant human semaphorin 4A (rhSema4A) at different concentrations and the expression of IL-6 was assayed by qRT-PCR. b Effects of rhSema4A, with or without lipopolysaccharide (LPS) (1 μg/ml) stimulation, on the secretion of IL-6 in the supernatant of RASFs was detected by ELISA. c After silencing Sema4A expression in RASFs, the IL-6 secretion was also detected by ELISA. d The expression of IL-6 in RASFs was detected by qRT-PCR after silencing Plexin B1, Plexin D1 or Tim-2, respectively. e The phosphorlation of Stat3, mitogen-activated protein kinase (MAPK), Met and NF-κB signaling in RASFs was analyzed by western blotting after treatment with rhSema4A (10 ng/ml) for the indicated periods. f IL-6 secretion in the supernatant of RASFs was determined by ELISA after various treatments. *P <0.05 and **P <0.01 (statistically significant differences)

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