PTEN does not influence DC maturation but importantly regulates T cell polarizing cytokines in vitro.
a BMDCs were either unstimulated or stimulated with the TLR ligands LPS (100 ng/ml) or CpG (5 μg/ml) and analyzed by flow cytometry. Plots show indicated surface markers on gated CD11c+ cells. Data are representative of at least three experiments. b Upregulation of CD86 and MHCII on CD11c+ cells in wt (n = 5) and myeloid pten
-/- (n = 5) after administration of LPS (75 μg) i.p. measured by flow cytometry. c Analysis of CFSE dilution of transferred OTII cells 3 days after transfer into wt or myeloid pten
-/- mice and immunization with LPS and ovalbumin i.v. d Analysis of levels of IL-6 and IL-12p40 in the supernatants of wt or myeloid pten
-/- BMDCs stimulated with LPS. BMDC bone marrow-derived dendritic cell, CFSE carboxyfluorescein succinimidyl ester, DC dendritic cell, IL interleukin, i.p. intraperitoneally, i.v. intravenously, LPS lipopolysaccharide, MHC major histocompatibility complex, PTEN phosphatase and tensin homolog, TLR Toll-like receptor, wt wild-type