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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Phosphatase and tensin homolog (PTEN) in antigen-presenting cells controls Th17-mediated autoimmune arthritis

Fig. 4

PTEN is not critically involved in APC migration and phenotypic maturation in vivo. ac Cells of draining LN from wt (n = 4) and myeloid pten -/- mice (n = 6) 2 weeks after induction of CIA and analyzed by flow cytometry. CD11c+ DCs (a) and CD11b+ macrophages (b) (shown as percent of total LN cells), and expression levels of CD80, CD86 and MHC II (mean fluorescence intensity, mfi) on CD11c+ cells or CD11b+ cells. c CD4+ T cells and CD4+CD25+ activated T cells (shown as percent of total CD4+ cells). Horizontal bars are means of each group ± s.e. d Spleen cells from wt or myeloid pten -/- mice of mock immunized (adjuvants only, left two bars) or after immunization with collagen (right two bars) were restimulated with 100 μg/ml collagen II in vitro and proliferation was quantified by H3Thymidine incorporation. Data are expressed as means ± s.d. APC antigen-presenting cell, CIA collagen-induced arthritis, DCs dendritic cells, LN lymph node, MHC major histocompatibility complex, PTEN phosphatase and tensin homolog

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