Fig. 6

Fluorescent images of undecalcified sections of the periarticular sites in tibiae. a Representative fluorescent images of the proximal tibiae. Scale bar represents 1 mm. b Representative fluorescent images of the secondary spongiosa of the tibiae. Scale bar represents 50 μm. c Magnified images of calcein double labeling of the trabecular bone. d Mineral apposition rate (MAR)/day. e Bone formation rate (BFR)/day in a bone surface reference. f BFR/year in a total tissue reference. g Trabecular thickness (Tb.Th). h Osteoblast surface per bone surface (Ob.S/BS). i Labeling surface at epiphysis. Data expressed as mean ± SD (n = 6) for each group. *p <0.05, **p <0.01 vs. Normal-vehicle; ^# p <0.05, ^## p <0.01 vs. CIA-vehicle. Normal-vehicle group, nonimmunized mice receiving vehicle (20 % DMSO); CIA-vehicle group, immunized mice receiving vehicle (20 % DMSO); CIA-9 mg OP3-4 group, immunized mice receiving 9 mg/kg/day OP3-4 peptide; CIA-18 mg OP3-4 group, immunized mice receiving 18 mg/kg/day OP3-4 peptide. CIA collagen-induced arthritis