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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Macitentan inhibits the transforming growth factor-β profibrotic action, blocking the signaling mediated by the ETR/TβRI complex in systemic sclerosis dermal fibroblasts

Fig. 1

Macitentan (MAC) inhibited both transforming growth factor beta (TGF-β) and endothelin-1 (ET-1) effects. Protein and gene expression of collagen 1 alpha 1 (Col1A1) and alpha smooth muscle actin (α-SMA) in fibroblasts (FBs). Under untreated (UT) conditions the basal expression of Col1A1 and α-SMA proteins was significantly higher in systemic sclerosis (SSc) FBs when compared to healthy control (HC) FBs. The TGF-β and ET-1 treatment induced a significant upregulation of both Col1A1 and α-SMA expression when compared to UT FBs and this increase was significantly higher in SSc FBs. MAC significantly blocked both TGF-β and ET-1 effects. Pictures are representative of all experiments. Protein bands were quantified by densitometry and the values are expressed as protein relative quantification/β actin relative quantification. **p = 0.0002, ***p = 0.0001

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