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Table 1 Affinity and potency of CDP7657 and hu5c8

From: CDP7657, an anti-CD40L antibody lacking an Fc domain, inhibits CD40L-dependent immune responses without thrombotic complications: an in vivo study

Assay CDP7657 Hu5c8
BIAcore affinity (KD) 7.9 pM 33 pM
CD40 binding (IC50) 44 ng/mL (490 pM) 44 ng/mL (293 pM)
CD40L-dependent B-cell activation (ICAM-1 expression) (IC50) 83 ng/mL (920 pM) 38 ng/mL (253 pM)*
  1. *Activity in this assay is very sensitive to the valency of anti-CD40L antibodies tested, and the IC50 for hu5c8 Fab is 977 ng/mL (nā€‰=ā€‰20). CD40L CD40 ligand, ICAM intercellular adhesion molecule 1