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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: O-linked N-acetylglucosamine glycosylation of p65 aggravated the inflammation in both fibroblast-like synoviocytes stimulated by tumor necrosis factor-α and mice with collagen induced arthritis

Fig. 3

An animal model of rheumatoid arthritis (RA). Two weeks after primary immunization, mice with collagen-induced arthritis (CIA) were treated with 20 mg ThiaMet-G via intraperitoneal injection every other day for 20 days. Mice were then sacrificed and immune cells isolated from the spleen; T cells were then isolated and analyzed using fluorescence-activated cell sorting. a ThiaMet-G significantly aggravated the severity of arthritis in treated compared to untreated mice with CIA. b Histopathological evaluation of joint sections revealed that synovial proliferation and bone destruction, but not inflammatory cell infiltration, were more severe in mice with CIA treated with ThiaMet-G than in untreated mice (original magnification × 100). c ThiaMet-G significantly increased the numbers of CD4+ IFN-γ+ T cells (8.5 % vs 6.8 %) and CD4+ IL-17+ T cells (17.0 % vs 14.3 %), but not CD4+ IL-4+ T cells or CD4+ FoxP3+ T cells, in treated mice with CIA compared to untreated controls, respectively (*P <0.05)

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