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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Recombinant human SIRT1 protects against nutrient deprivation-induced mitochondrial apoptosis through autophagy induction in human intervertebral disc nucleus pulposus cells

Fig. 1

Deacetylation potential of recombinant human silent mating type information regulator 2 homolog 1 (rhSIRT1) introduced into human nucleus pulposus cells. a Western blot analysis of acetylated and total p53, and acetylated and total nuclear factor κB (NF-κB) in nucleus pulposus cells from intervertebral discs (IVDs) of Pfirrmann grades II and IV after 72 h of treatment with 10 μM rhSIRT1 under normal nutrition (N) conditions with 10 % (v/v) fetal bovine serum (FBS) or low nutrition (LN) conditions with 1 % (v/v) FBS. Cells introduced with a nonspecific protein, R-phycoerythrin, were used as the control. α-Tubulin was used as the loading control. b Ratio of acetylated p53 to total p53 and acetylated NF-κB to total NF-κB. Data are expressed as the mean ± standard deviation (n = 1 for cells from grade II IVD and n = 4 for cells from grade IV IVDs). *P < 0.05; P < 0.01, three-way analysis of variance with the Tukey–Kramer post hoc test

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