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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Recombinant human SIRT1 protects against nutrient deprivation-induced mitochondrial apoptosis through autophagy induction in human intervertebral disc nucleus pulposus cells

Fig. 2

Effect of recombinant human silent mating type information regulator 2 homolog 1 (rhSIRT1) on the number of human nucleus pulposus (NP) cells. a Morphological appearance of NP cells from intervertebral discs (IVDs) of Pfirrmann grade II and IV after 14 days of treatment with 10 μM rhSIRT1 or 5 mM 3-methyladenine (3-MA) under normal nutrition (N) conditions with 10 % (v/v) fetal bovine serum (FBS) or low nutrition (LN) conditions with 1 % (v/v) FBS (bars, 250 μm). b Changes in the number of NP cells from IVDs of Pfirrmann grades II and IV after 0, 3, 7, and 14 days of treatment. Data are expressed as the mean ± standard deviation (n = 5). *P < 0.05; P < 0.01, three-way analysis of variance with the Tukey–Kramer post hoc test

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