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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Recombinant human SIRT1 protects against nutrient deprivation-induced mitochondrial apoptosis through autophagy induction in human intervertebral disc nucleus pulposus cells

Fig. 5

Effect of recombinant human silent mating type information regulator 2 homolog 1 (rhSIRT1) on apoptosis mediated through the mitochondrial pathway in human nucleus pulposus cells. a Western blot analysis of Bcl-2-associated X protein (Bax), Bcl-2, full-length caspases 3 and 9, and cleaved caspases 3 and 9 in nucleus pulposus cells from intervertebral discs of Pfirrmann grade IV after 72 h of treatment with 10 μM rhSIRT1 or 5 mM 3-methyladenine (3-MA) under normal nutrition (N) conditions with 10 % (v/v) fetal bovine serum (FBS) or low nutrition (LN) conditions with 1 % (v/v) FBS. α-Tubulin was used as a loading control. b Ratio of Bax to Bcl-2 and cleaved caspases 3 and 9 to tubulin. Data are expressed as the mean ± standard deviation (n = 4). *P < 0.05; P < 0.01, three-way analysis of variance with the Tukey–Kramer post hoc test

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