Culture groups | Culture conditions |
---|---|
Group N | DMEM with 10Â % (v/v) FBS |
Group LN | DMEM with 1Â % (v/v) FBS |
Group LN + SIRT1 | DMEM with 1 % (v/v) FBS + 10 μM rhSIRT1 |
Group LN + SIRT1 + 3-MA | DMEM with 1 % (v/v) FBS + 10 μM rhSIRT1 + 5 mM 3-MA |