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Table 2 Experimental groups

From: Recombinant human SIRT1 protects against nutrient deprivation-induced mitochondrial apoptosis through autophagy induction in human intervertebral disc nucleus pulposus cells

Culture groups Culture conditions
Group N DMEM with 10 % (v/v) FBS
Group LN DMEM with 1 % (v/v) FBS
Group LN + SIRT1 DMEM with 1 % (v/v) FBS + 10 μM rhSIRT1
Group LN + SIRT1 + 3-MA DMEM with 1 % (v/v) FBS + 10 μM rhSIRT1 + 5 mM 3-MA
  1. Abbreviations: DMEM Dulbecco’s modified Eagle’s medium, FBS fetal bovine serum, LN low nutrition, 3-MA 3-methyladenine, N normal nutrition, rhSIRT1 recombinant human silent mating type information regulator 2 homolog 1, SIRT1 silent mating type information regulator 2 homolog 1