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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Histone deacetylase inhibition activates Nrf2 and protects against osteoarthritis

Fig. 1

Nrf2-KO mice display more severe articular cartilage damage in osteoarthritis (OA) animal models. a Nrf2-KO and wild-type (WT) mice were subject to monosodium iodoacetate (MIA) OA. Mouse joint sections were stained with Safranin O/Fast Green. Representative images are shown (n = 10 mice per group). Lower panels show amplifications of upper panel insets. Yellow arrows point to the damage-affected cartilage areas. b Summed scores of femur and tibia damage in WT and Nrf2-KO mice in the MIA model. *p < 0.05, **p < 0.01. c Maximum scores of femur and tibia damage from WT and Nrf2-KO mice of MIA model. *p < 0.05, ** p < 0.01. d Nrf2-KO and WT mice were subject to DMM surgery. Mouse joint sections were stained with Safranin O/Fast Green and the representative images were shown (n = 10 mice per group). Lower panels were the amplifications of windows from upper panels. The yellow arrows indicate the damage-affected cartilage areas. e Summed scores of femur and tibia damage from WT and Nrf2-KO mice of DMM model. *p < 0.05, **p < 0.01. f Maximum scores of femur and tibia damage from WT and Nrf2-KO mice of DMM model. *p < 0.05, ** p < 0.01. g PCR genotyping of WT and Nrf2-KO mice on mouse tail DNA. h Western blot analysis of heme oxygenase 1 (HO-1) and NAD(P)H:quinine oxidoreductase 1 (NQO1) from mouse joint tissues of WT and Nrf2-KO mice (three mice in each group). i Quantitative analysis of HO1 and NQO1 protein levels. *p < 0.05, **p < 0.01. j Western blot analysis of matrix metalloproteinases MMP-1, MMP-3, and MMP-13 from the joint tissues of WT and Nrf2-KO mice (three mice in each group). k Quantitative analysis of MMP-1, MMP-3, and MMP-13 protein levels. *p < 0.05. KO knockout, Nrf2 nuclear factor (erythroid-derived 2)-like 2

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