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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Enhanced activity of NLRP3 inflammasome in peripheral blood cells of patients with active rheumatoid arthritis

Fig. 3

Induction of nucleotide binding domain and leucine-rich repeat pyrin 3 domain (NLRP3)-inflammasome related proteins in peripheral whole blood cells, upon sequential stimulation with lipolysaccharide (LPS) (250 pg/mL, 2 h) and ATP (5 mM, 20 min), in healthy individuals and patients with rheumatoid arthritis (RA). Western blots were performed in freshly isolated whole blood cells without (−) or with (+) LPS + ATP treatment from healthy controls (a) (n = 7) and patients with active RA (b) (n = 7). Protein densitometry was assessed using Image J program and the intensity of the protein of interest was normalized to that of β-actin (c-h). HC healthy controls

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