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Table 1 Queries used to identify different cell populations using our database storing results from ImmPort-FLOCK

From: Reanalysis of the Rituximab in ANCA-Associated Vasculitis trial identifies granulocyte subsets as a novel early marker of successful treatment

Cell populations Queries used
Total lymphocytes FSC ≤ 2 AND SSC = 1
CD1c + lymphocytes FSC ≤ 2 AND SSC = 1 AND CD1c ≥ 2
CD5+ lymphocytes FSC ≤ 2 AND SSC = 1 AND CD5 ≥ 2
CD19+ lymphocytes FSC ≤ 2 AND SSC = 1 AND CD19 ≥ 2
CD21+ lymphocytes FSC ≤ 2 AND SSC = 1 AND CD21 ≥ 2
CD23+ lymphocytes FSC ≤ 2 AND SSC = 1 AND CD23 ≥ 2
Total monocytes FSC ≥ 3 AND SSC ≤ 2
Total granulocytes ((FSC ≥ 3 AND SSC ≥ 3) OR (FSC ≤ 2 AND SSC ≥ 2))
Hypergranular granulocytes FSC ≥ 1 AND SSC = 4
Hypogranular granulocytes (FSC ≤ 2 AND SSC = 3) OR (FSC ≤ 2 AND SSC = 2) OR (FSC ≥ 3 AND SSC = 3)
  1. CD cluster of differentiation, FSC forward scatter, ImmPort-FLOCK, Immunology Database and Analysis Portal flow cytometry clustering without K, SSC side scatter