Table 1 Queries used to identify different cell populations using our database storing results from ImmPort-FLOCK
Cell populations | Queries used |
|---|---|
Total lymphocytes | FSC ≤ 2 AND SSC = 1 |
CD1c + lymphocytes | FSC ≤ 2 AND SSC = 1 AND CD1c ≥ 2 |
CD5+ lymphocytes | FSC ≤ 2 AND SSC = 1 AND CD5 ≥ 2 |
CD19+ lymphocytes | FSC ≤ 2 AND SSC = 1 AND CD19 ≥ 2 |
CD21+ lymphocytes | FSC ≤ 2 AND SSC = 1 AND CD21 ≥ 2 |
CD23+ lymphocytes | FSC ≤ 2 AND SSC = 1 AND CD23 ≥ 2 |
Total monocytes | FSC ≥ 3 AND SSC ≤ 2 |
Total granulocytes | ((FSC ≥ 3 AND SSC ≥ 3) OR (FSC ≤ 2 AND SSC ≥ 2)) |
Hypergranular granulocytes | FSC ≥ 1 AND SSC = 4 |
Hypogranular granulocytes | (FSC ≤ 2 AND SSC = 3) OR (FSC ≤ 2 AND SSC = 2) OR (FSC ≥ 3 AND SSC = 3) |