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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Bach1 deficiency reduces severity of osteoarthritis through upregulation of heme oxygenase-1

Fig. 1

Expression of heme oxygenase-1 (HO-1) in articular cartilages from wild-type (WT) mice and Bach1-/- mice. Primary articular chondrocytes were isolated from mouse femoral head cartilage (n = 4 per group) at the age of 1 month. a Real-time PCR assay for Hmox1. b Western blot analysis of HO-1 protein (n = 4). c Evaluation of the phenotype of articular chondrocytes in Bach1-/- mice. Expression of cartilage-associated genes was determined by real-time PCR assay (n = 4 per group). d Comparison of HO-1 expression in articular cartilage from 22-month-old wild-type mice and Bach1-/- mice (n = 4 per group). Knee joints were assessed by immunohistochemistry using anti-HO-1 antibody. Original magnification × 10 (left; scale bars 200 μm, and × 20 (right; scale bars 100 μm). Values in a, c and d were expressed as means ± SD. Statistical analysis was performed with the Mann–Whitney U test; **P <0.01

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