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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Bach1 deficiency reduces severity of osteoarthritis through upregulation of heme oxygenase-1

Fig. 5

The involvement of heme oxygenase-1 (HO-1) in changes observed in Bach1-/- articular chondrocytes. Small interfering HO-1 (siHO-1) or control siRNA (10 uM) was transfected into chondrocytes from wild-type (WT) and Bach1-/- mice at 1 month of age (n = 4 per group). a The expression of superoxide dismutase 2 (SOD2) protein was detected by immunoblot analysis. Values are the mean ± SD. Statistical analysis was performed with the Steel–Dwass test; *P <0.05 versus wild-type chondrocytes. b The expression of Mmp-13 and Adamts-5 in Bach1-/- chondrocytes with IL-1β (1 ng/ml) for 24 h. Values are the mean ± SD. Statistical analysis was performed with the Steel test; *P <0.05 versus wild-type chondrocytes (-IL); # P <0.05 versus Bach1-/- chondrocytes (-IL). c Caspase-3/7 was detected in wild-type and Bach1-/- mouse chondrocytes with or without the oxidant tert-butyl hydroperoxide (t-BHP) (200 uM) for 5 h. Values are the mean ± SD. Statistical analysis was performed with the Steel–Dwass test; *P <0.05, **P <0.01 versus wild-type chondrocytes with t-BHP; ## P <0.01 versus Bach1-/- chondrocytes treated with t-BHP. GAPDH glyceraldehyde–3–phosphate dehydrogenase

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