Fig. 2

Splenic cellularity and activation profiles of Cre ^LysM Casp8 ^fl/fl mice are not drastically altered. Splenocytes from 6–8-month-old (aged) female Casp8 ^fl/fl (control) and Cre ^LysM Casp8 ^fl/fl mice (n ≥ 4) were analyzed by flow cytometry. a Number of CD11b⁺F4/80⁻Ly6G⁺ neutrophils, Ly6C^high and Ly6C^low CD11b⁺F4/80⁺ cells, and CD11b⁻F4/80⁺ red pulp macrophages. b Representative fluorescence-activated cell sorting (FACS) plots of CD11b⁺F4/80⁺Ly6C^high and CD11b⁺F4/80⁺Ly6C^low splenocytes displaying levels of surface activation marker expression. c Number of CD11c⁺CD8⁻ and CD11c⁺CD8⁻ conventional DCs and CD11c^intermediatePDCA-1⁺B220⁺ plasmacytoid DCs. d Representative FACS plots of CD11c⁺CD8⁻ and CD11c⁺CD8⁻ conventional DCs and CD11c^intermediatePDCA-1⁺B220⁺ plasmacytoid DCs displaying levels of surface activation marker expression. e Total B-cell (CD11c⁻B220⁺) numbers. f B-cell subsets: follicular (FO; CD19⁺CD21/35⁺CD23⁺), marginal zone (MZ; CD19⁺CD21/35⁺CD23^low), transitional 1 (T1; B220⁺AA4.1⁺CD23⁻), transitional 2 (T2; B220⁺AA4.1⁺CD23⁺), plasmablasts (PB; CD19⁺B220^lowCD138⁺CD21/35⁻CD23⁻), and plasma cells (PC; CD19⁺B220⁺CD138⁺CD21/35⁻CD23⁻). g Representative FACS plots from B cells displaying levels of surface activation and maturation marker expression. h Representative FACS plots and quantitative graphs of total CD4⁺ and CD8⁺ T-cell numbers, naïve (CD44⁻CD62L⁺), central memory (CD44⁺CD62L⁺) and activated (CD44⁺CD62L⁻) CD4⁺ and CD8⁺ T-cell numbers, and CD4⁻CD8⁻CD3⁺B220⁺ double-negative T-cell numbers. i Representative FACS plots and quantitative graphs depicting activated CD4⁺ and CD8⁺ T cells displaying levels of surface activation marker expression. j CD4⁺CD25⁺Foxp3⁺ regulatory T-cell numbers. Data are represented as mean ± SD and were compared by Mann–Whitney U test: **p < 0.005, ***p < 0.0005. k Bead-separated CD11b⁺ cells incubated with ovalbumin were cocultured with B6.CD45.1/OT-II/RAG ^−/− CD4⁺ T cells at various ratios with or without CpG. Data are represented as mean ± SD of biological triplicates, and experiments were repeated twice. Data were compared by Mann–Whitney U test: *p < 0.05; **p < 0.005. PDCA-1 plasmacytoid dendritic cell antigen 1