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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Conditional deletion of caspase-8 in macrophages alters macrophage activation in a RIPK-dependent manner

Fig. 2

Splenic cellularity and activation profiles of Cre LysM Casp8 fl/fl mice are not drastically altered. Splenocytes from 6–8-month-old (aged) female Casp8 fl/fl (control) and Cre LysM Casp8 fl/fl mice (n ≥ 4) were analyzed by flow cytometry. a Number of CD11b+F4/80Ly6G+ neutrophils, Ly6Chigh and Ly6Clow CD11b+F4/80+ cells, and CD11bF4/80+ red pulp macrophages. b Representative fluorescence-activated cell sorting (FACS) plots of CD11b+F4/80+Ly6Chigh and CD11b+F4/80+Ly6Clow splenocytes displaying levels of surface activation marker expression. c Number of CD11c+CD8 and CD11c+CD8 conventional DCs and CD11cintermediatePDCA-1+B220+ plasmacytoid DCs. d Representative FACS plots of CD11c+CD8 and CD11c+CD8 conventional DCs and CD11cintermediatePDCA-1+B220+ plasmacytoid DCs displaying levels of surface activation marker expression. e Total B-cell (CD11cB220+) numbers. f B-cell subsets: follicular (FO; CD19+CD21/35+CD23+), marginal zone (MZ; CD19+CD21/35+CD23low), transitional 1 (T1; B220+AA4.1+CD23), transitional 2 (T2; B220+AA4.1+CD23+), plasmablasts (PB; CD19+B220lowCD138+CD21/35CD23), and plasma cells (PC; CD19+B220+CD138+CD21/35CD23). g Representative FACS plots from B cells displaying levels of surface activation and maturation marker expression. h Representative FACS plots and quantitative graphs of total CD4+ and CD8+ T-cell numbers, naïve (CD44CD62L+), central memory (CD44+CD62L+) and activated (CD44+CD62L) CD4+ and CD8+ T-cell numbers, and CD4CD8CD3+B220+ double-negative T-cell numbers. i Representative FACS plots and quantitative graphs depicting activated CD4+ and CD8+ T cells displaying levels of surface activation marker expression. j CD4+CD25+Foxp3+ regulatory T-cell numbers. Data are represented as mean ± SD and were compared by Mann–Whitney U test: **p < 0.005, ***p < 0.0005. k Bead-separated CD11b+ cells incubated with ovalbumin were cocultured with B6.CD45.1/OT-II/RAG −/− CD4+ T cells at various ratios with or without CpG. Data are represented as mean ± SD of biological triplicates, and experiments were repeated twice. Data were compared by Mann–Whitney U test: *p < 0.05; **p < 0.005. PDCA-1 plasmacytoid dendritic cell antigen 1

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