Differences in CCR6−CD4+ T cell subpopulations and Tregs between ACPA+ patients and matched ACPA− patients. a Gating strategy for the identification of peripheral blood Th1, Th2 and CCR4/CXCR3 DP cell subpopulations. CCR6− cells were gated on CD4+CD45RO+CD25− T cells. b Gating strategy to identify Treg (CD25hiFOXP3+) cells within the memory CD4+ T cell population. Cells were gated on the total lymphocyte population. c Proportions of the indicated CD4+ T cell subpopulations within the total memory CD4+ T cell population of 27 ACPA+ and 27 ACPA− patients with RA. For statistical analysis Wilcoxon matched-pairs signed-ranks test was performed (* = p < 0.05).