Fig. 3

Differences in CCR6⁻CD4⁺ T cell subpopulations and Tregs between ACPA⁺ patients and matched ACPA⁻ patients. a Gating strategy for the identification of peripheral blood Th1, Th2 and CCR4/CXCR3 DP cell subpopulations. CCR6⁻ cells were gated on CD4⁺CD45RO⁺CD25⁻ T cells. b Gating strategy to identify Treg (CD25^hiFOXP3⁺) cells within the memory CD4⁺ T cell population. Cells were gated on the total lymphocyte population. c Proportions of the indicated CD4⁺ T cell subpopulations within the total memory CD4⁺ T cell population of 27 ACPA⁺ and 27 ACPA⁻ patients with RA. For statistical analysis Wilcoxon matched-pairs signed-ranks test was performed (* = p < 0.05).