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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Analyzing pathogenic (double-stranded (ds) DNA-specific) plasma cells via immunofluorescence microscopy

Fig. 1

Analysis of double-stranded deoxyribonucleic acid (dsDNA)-specific plasma cells (PCs) by histology and ELISpot. a Kryosections from bone marrows and spleens of autoimmune (NZB/W) and non-autoimmune (C57BL/6) mice were stained with anti-immunoglobulin light chain kappa (IgL) (PCs, green) and fluorochrome-labeled dsDNA (red, - > dsDNA-specific PCs = yellow) and analyzed by confocal laser scanning microscopy. b For comparison standard enzymatic ELISpot (dsDNA of all isotypes) and immunofluorescence ELISpot (dsDNA red; IgG, IgA and IgM green) are depicted. For immunofluorescence ELISpot only 5 % of original cell numbers were seeded to enable counting of total PCs in parallel. c Numbers of dsDNA-specific PCs acquired by histology and ELISpot. With both methods, dsDNA PCs could be identified in autoimmune (NZB/W) but none to very few in non-autoimmune (C57BL/6) mice. d In probes from the same NZB/W mice, numbers of dsDNA-specific PCs were assessed by histology and ELISpot. Data in spleen and bone marrow correlated significantly

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