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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Analyzing pathogenic (double-stranded (ds) DNA-specific) plasma cells via immunofluorescence microscopy

Fig. 2

Parallel analysis of pathogenic (double-stranded deoxyribonucleic acid (dsDNA)-specific) and protective (ovalbumin (Ova)-specific) plasma cells (PCs), discrimination of dsDNA-specific PCs according to their immunoglobulin (Ig) class (IgA, IgG and IgM) and identification of autoreactive long-lived plasma cells (LLPCs) and short-lived plasma cells (SLPCs). a Kryosections from spleen of autoimmune mice (NZB/W) - 5 days post secondary Ova immunization - were stained with anti-Ig light chain kappa (IgL) (PCs, green), fluorochrome-labeled dsDNA (red, - > dsDNA-specific PCs = yellow) and fluorochrome-labeled Ova (blue, Ova-specific PCs = turquoise). PCs were either specific for dsDNA, Ova or an unknown antigen but no false double-positive dsDNA/Ova PCs occurred. For comparison, a figure of an immunofluorescence ELISpot identifying Ova (green) and dsDNA (red) antibody-secreting cells is depicted. b Kryosections from spleen of autoimmune (NZB/W) mice were stained with fluorochrome-labeled dsDNA (white), anti-IgA (red), anti-IgG (green), anti-IgM (blue). dsDNA-specific PCs were either IgM, IgG or IgA positive. The figures below depict dsDNA PCs of the IgM (left) and IgG (right) class acquired by ELISpot c Autoimmune (BcN/LmoJ) mice were fed EdU for 2 weeks. Kryosections from spleen were stained with click-it® EdU kit (EdU positive = white), IgL (PCs, green), fluorochrome-labeled dsDNA (red, - > dsDNA-specific PCs = yellow). dsDNA-specific SLPC incorporated EdU (lower selection, indicated by arrow in magnification, white-colored nucleus) during proliferation while dsDNA-specific LLPCs did not (upper selection, indicated by arrow in magnification, blank nucleus)

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