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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: HIF-2α-induced chemokines stimulate motility of fibroblast-like synoviocytes and chondrocytes into the cartilage-pannus interface in experimental rheumatoid arthritis mouse models

Fig. 4

HIF-2α mediates IL-1β-induced chemokine expression in chondrocytes. a RT-PCR and b qRT-PCR analyses of mRNA in chondrocytes treated with IL-1β for 24 hours (n = 5). c RT-PCR and western blot analyses of the indicated molecules in primary cultures of chondrocytes isolated from WT (Epas1 +/+) or Epas1 +/− mice treated with IL-1β for 24 hours. RT-PCR was carried out to detect transcript levels of Epas1, Cxcl1, Cxcl2, and Ccl5. Secreted chemokine proteins were examined via western blot. d Immunostaining of HIF-2α, CXCL1, CXCL2, and CCL5 in cartilage tissue of WT and Epas1 +/− DBA1/J mice with CIA. Values presented as mean ± standard error of the mean (*P <0.05, **P <0.01). Scale bar: 50 μm. CIA collagen-induced arthritis, DAPI 4′,6-diamidino-2-phenylindole, HIF hypoxia-inducible factor, IL interleukin

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