Fig 1

Immunoglobulin production by activated human B lymphocytes in vitro is suppressed by Acthar. IgG (top panel) and IgM (bottom panel) were measured in supernatants from human peripheral B cells cultured for 6 days under basal conditions, with added IL-4/CD40L alone, or with IL-4/CD40L plus added Acthar at ACTH analog concentrations of approximately 0.124, 1.24, or 2.49 μM (stepped bars). Parallel cultures contained IL-4/CD40L plus matched volumes of placebo. Supernatants were harvested and assayed for IgG or IgM concentration and results analyzed by ANOVA (Kruskal–Wallis test, p <0.0001 for IgG; p = 0.0551 for IgM). **Significantly different from IL-4/CD40L-stimulated samples by Dunn’s post test at p <0.01. *Significantly different from IL-4/CD40L-stimulated samples by Dunn’s post test at p <0.05. Results shown are mean ± SEM for eight independent experiments. Acthar H.P. Acthar Gel®, CD40L CD40 ligand, Ig immunoglobulin, IL interleukin