Fig. 3From: Direct effects of HP Acthar Gel® on human B lymphocyte activation in vitroViability of B lymphocytes in vitro is unaffected by Acthar. The percentage of live cells (negative for staining) was assessed in human peripheral B cells cultured for 6 days under basal conditions, with added IL-4/CD40L alone, or with IL-4/CD40L plus added Acthar at ACTH analog concentrations of approximately 0.124, 1.24, or 2.49 μM. Parallel cultures contained IL-4/CD40L plus volumes of placebo at equal volumes. At the end of the culture period cells were stained with Live/Dead stain as described in Methods. Cells were analyzed by flow cytometry. Statistical analysis (ANOVA) revealed none of the treatment values to be significantly different from IL-4/CD40L-stimulated sample. Results shown are mean ± SEM for six independent experiments. Acthar H.P. Acthar Gel®, CD40L CD40 ligand, FITC fluorescein isothiocyanate, IL interleukinBack to article page