Fig. 4
From: Direct effects of HP Acthar Gel® on human B lymphocyte activation in vitro
Expression of AICDA mRNA in activated human B lymphocytes in vitro is suppressed by Acthar. Expression of AICDA mRNA was assessed in human peripheral B cells cultured a for 1 day or b for 6 days under basal conditions, with added IL-4/CD40L alone, or with IL-4/CD40L plus added Acthar at ACTH analog concentrations of approximately 0.124, 1.24, or 2.49 μM. Parallel cultures contained IL-4/CD40L plus placebo at matched volumes. mRNA was isolated and quantitated by RT/PCR as described in Methods. Data were analyzed by ANOVA (p = 0.0002 on day 1 and p = 0.9973 on day 6). *Significantly different from IL-4/CD40L-stimulated samples by Tukey’s post test at p <0.05; **Significantly different from IL-4/CD40L-stimulated samples by Tukey’s post test at p <0.01. Results shown are mean ± SEM of six independent experiments at 1 day and of seven independent experiments at 6 days. Acthar H.P. Acthar Gel®, AICDA activation-induced cytidine deaminase, CD40L CD40 ligand, IL interleukin