Fig. 5
From: Excessive reactive oxygen species are therapeutic targets for intervertebral disc degeneration
Treatment of α-tocopherol significantly abolishes the catabolic effect of ROS. a, b Real-time RT-PCR analysis. Treatment with α-tocopherol significantly abolished the H2O2-mediated a or BSO-mediated b induction of TNFα and COX-2 mRNA expression, and reduction of aggrecan. H2O2-mediated induction of MMP-3 expression was not significant, but tended to be decreased. Data presented as mean ± SD of five independent experiments performed in triplicate (n = 5); *p <0.05. BSO buthionine sulfoximine, COX cyclooxygenase, H 2 O 2 hydrogen peroxide, HPRT hypoxanthine phosphoribosyl transferase, MMP matrix metalloprotease, ns not significant, TNF tumor necrosis factor