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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Inhibition of angiogenesis by platelets in systemic sclerosis patients

Fig. 5

Systemic sclerosis (SSc) platelet supernatant stimulates fibroblast proliferation and α-smooth muscle actin (α-SMA) expression. a Human fibroblast proliferation was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay after 24 h culture alone [non-stimulated (NS)] or with transforming growth factor (TGF)-β as well as with platelet releasates from patients with SSc and from healthy control subjects. The rightmost bar is a negative control and corresponds to normal platelets. Higher proliferation was observed after incubation with SSc platelet supernatants than with healthy control supernatants (*p < 0.05, Mann–Whitney U test). b In the same experiment, α-SMA expression was evaluated in fibroblasts. Higher α-SMA expression was found in fibroblasts incubated with SSc platelet releasates than with healthy control releasates (*p < 0.05, analysis of variance). Expression was determined by performing real-time polymerase chain reactions using TaqMan assays

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