Fig. 4From: Antibodies against High Mobility Group Box protein-1 (HMGB1) versus other anti-nuclear antibody fine-specificities and disease activity in systemic lupus erythematosusCellular localization of high mobility box protein-1 (HMGB1). Indirect immunofluorescence microscopy images. a Cytoplasmic/extra-chromosomal HEp-2 cell immunofluorescence after incubation with rabbit IgG anti-HMGB1 followed by FITC-conjugated anti-rabbit IgG. b Negative control HEp-2 cells incubated with fluorescein-isothiocyanate (FITC)-conjugated anti-rabbit IgG alone. c Hepatocyte nuclear fluorescence after incubation of rat liver cryostat section with rabbit anti-HMGB1 and FITC anti-rabbit IgGBack to article page