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Fig. 6 | Arthritis Research & Therapy

Fig. 6

From: Soluble PD-1 aggravates progression of collagen-induced arthritis through Th1 and Th17 pathways

Fig. 6

PD-1-Fc promotes the expansion of Th1 and Th17 cells in vitro. a The proliferation rates of spleen lymphocytes from collagen-induced arthritis (CIA) mice cultured with type II collagen (CII) or CD3 and PD-1-Fc were determined by Cell Counting Kit-8 assay. Bars show the mean and standard error of the mean. *p < 0.05 versus control. b and c Cytokine levels in supernatants of spleen lymphocytes from CIA mice that were stimulated with CII or CD3 antibody in the presence of PD-1-Fc, as determined by cytometric bead array. Data are representative of three individual sample analyses. Bars show the mean and standard deviation (SD). *p < 0.05. d Percentage of Th1, Th2, and Th17 cells in CD4+ T cells from spleen lymphocytes of CIA mice with or without PD-1-Fc induction. Percentages were determined by intracellular staining. Data are representative of three individual sample analyses. Bars show the mean and SD. *p < 0.05 versus control. IFN interferon, IgG immunoglobulin G, IL interleukin, OD optical density, PD-1 programmed cell death 1 protein, TNF tumor necrosis factor

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