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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Simultaneous inhibition of JAK and SYK kinases ameliorates chronic and destructive arthritis in mice

Fig. 2

Efficacy of Janus kinase (JAK) + spleen tyrosine kinase (SYK) inhibition as a curative treatment in glucose-6-phosphate isomerase (G6PI)-induced arthritis. DBA/1 mice were immunized with G6PI + complete Freund’s adjuvant on day 0, after T regulatory cell depletion with anti-CD25 Ab (days −8 and −11). Treatments were orally administered daily from day 12 (red dotted line). Samples were obtained on day 31. a Time course for arthritis scores and resulting areas under these score curves (AUC). b Detailed clinical score by paw segment at day 31. c Cytokine secretion by splenocytes stimulated in vitro with 20 μg/ml recombinant human G6PI for 72 h. d Cytokine expression in splenocytes. e Numbers of CD19+MHCII+CD86+ activated B cells in spleens. f Levels of G6PI-specific IgGs in plasma. g and h Data and representative histological sections of tarsal joints (bar 200 μM). Graphs show mean (+ standard error of the mean) for 3–7 mice/group, *p <0.05 versus vehicle control. N naive, V/Veh vehicle, Pred/Predni prednisolone

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