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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: The interaction of lubricin/proteoglycan 4 (PRG4) with toll-like receptors 2 and 4: an anti-inflammatory role of PRG4 in synovial fluid

Fig. 1

Binding of recombinant human proteoglycan-4 (rhPRG4) and native human PRG4 (nhPRG4) to recombinant toll-like receptors 2 and 4 (TLR2 and TLR4) using a direct binding enzyme linked immunosorbent assay. PRG4 concentrations are reported in μg/mL and pmoles per well, based on a predicted molecular weight of 240 kDa. TLR2 and TLR4 are expressed as pmoles coated per well, based on predicted molecular weights of 66 and 70 kDa, respectively. The 450 nm absorbance values were normalized to non-specific binding. Data represent the mean ± SD from four independent experiments. Dashed line represents non-specific binding (background). a Concentration-dependent binding of rhPRG4 and nhPRG4 to immobilized TLR2. rhPRG4 exhibited a concentration-dependent binding at 50, 10 and 1 μg/mL. nhPRG4 exhibited a concentration-dependent binding at 50 and 10 μg/mL; *p <0.001. b Concentration-dependent binding of rhPRG4 and nhPRG4 to immobilized TLR4. rhPRG4 and nhPRG4 exhibited concentration-dependent binding at 50 and 10 μg/mL; *p <0.001

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