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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: The interaction of lubricin/proteoglycan 4 (PRG4) with toll-like receptors 2 and 4: an anti-inflammatory role of PRG4 in synovial fluid

Fig. 2

Association of recombinant human proteoglycan-4 (rhPRG4) with toll-like receptor-2 and 4 (TLR2 and TLR4) expressing human embryonic kidney (TLR2-HEK and TLR4-HEK) cells. Data represent the mean ± SD from four independent experiments. a Representative flow cytometry scatter plots of rhodamine-labeled rhPRG4 (20 μg/mL) association with TLR2-HEK and TLR4-HEK cells following incubation for 12 and 24 h. A threshold was set at red fluorescence intensity = 10. Cell-associated fluorescence higher than 10 was considered positive. Following incubation of rhodamine-rhPRG4 with TLR2-HEK and TLR4-HEK cells for 12 or 24 h, cell association was observed. b Quantitative analysis of the percentage of positive TLR2-HEK and TLR4-HEK cells following incubation with rhodamine-rhPRG4 (20 μg/mL) for 12 and 24 h. rhPRG4 was significantly associated with TLR2-HEK and TLR4-HEK at 24 h compared to 12 h and controls. The 12-h association between rhPRG4 and TLR2-HEK cells was significantly higher than with TLR4-HEK cells and both were higher than control; *p <0.001, ** p <0.05

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