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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: The interaction of lubricin/proteoglycan 4 (PRG4) with toll-like receptors 2 and 4: an anti-inflammatory role of PRG4 in synovial fluid

Fig. 4

Activation of toll-like receptors 2 and 4 (TLR2 and TLR4) expressing human embryonic kidney (TLR2-HEK and TLR4-HEK) cells by synovial fluids (SF) from subjects with no joint arthropathy (normal; n = 3), patients with osteoarthritis (OA; n = 8) and patients with rheumatoid arthritis (RA; n = 5) and effect of native human proteoglycan-4 (nhPRG4) treatment. SF samples were incubated with TLR2- or TLR4-HEK cells (3.75 % dilution) at 37 °C for 48 h. Absorbance values across groups were normalized to untreated cells (control). Data represent the mean SD from three independent experiments. a Activation of TLR2 and TLR4 by normal, OA and RA SF. Normal SF did not significantly activate TLR2 or TLR4 compared to control. OA SF and RA SF significantly activated TLR2 and TLR4 compared to normal SF and control. RA SF significantly activated TLR2 compared to OA SF; *p <0.001. b Impact of nhPRG4 treatment (100 μg/mL) on Pam3CSK4, OA SF and RA SF-induced activation of TLR2. nhPRG4 treatment inhibited Pam3CSK4, OA SF and RA SF-induced TLR2 activation; *p <0.001. c Impact of nhPRG4 treatment (150 μg/mL) on lipopolysaccharide (LPS), OA SF and RA SF-induced activation of TLR4. nhPRG4 treatment inhibited LPS, OA SF and RA SF-induced TLR4 activation; *p <0.001

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