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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: The HLA-B*35 allele modulates ER stress, inflammation and proliferation in PBMCs from Limited Cutaneous Systemic Sclerosis patients

Fig. 2

Heatmap showing the expression of gene clusters. PBMCs were isolated from healthy control and transduced with 0.1-0.5-1 MOI of lentivirus encoding HLA-B*35, HLA-B*8, or control lentivirus for 72 h. The global changes in gene expression were investigated by Illumina HT-12 arrays (Illumina Inc, San Diego, CA, USA). Among genes downregulated by HLA-B*35 lentivirus compared to HLA-B*8, we observed genes related to complement (C1QB, C1QC), cell cycle (CDNK1A), and apoptotic (Bax, Gadd45) pathways. Genes with increased expression levels were related to proliferation (FYN, ATM), inflammation (HMGB1), and ER stress/UPR (HSPA1A and DNAJB1). Expression values above the mean are indicated in dark blue, those below the mean are indicated in light blue. PBMCs, peripheral blood mononuclear cells, MOI multiplicity of infection, ER endoplasmic reticulum, UPR unfolded protein response

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