Fig. 4

R848-stimulated proliferation, survival, and Ig production by B cell subsets from pristane-treated mice. Splenic naïve B cells (NB), switched B memory cells (sMB), switched plasmablasts (sPB) and plasma cells (PC) were flow-sorted from pristane-treated or PBS-treated mice. a Sorted B cell subsets from PBS-treated mice were cultured for 3 days with R848. Proliferation was assessed by flow cytometry of carboxyfluorescein diacetate succinimidyl ester (CFSE)-loaded cells (values are geometric means). b Sorted B cell subsets from PBS-treated mice were cultured for 24 h in the presence or absence of R848 and cell death was assessed by 7-AAD staining (flow cytometry). Data are representative of two separate experiments. c Sorted B cell subsets from pristane treated mice were stimulated with R848 or PBS for 10 days. IgG and IgM were measured in culture supernatants by ELISA. Data are representative of three separate experiments: *p <0.05; ***p <0.001 (paired Student t test)