Fig. 5

R848 preferentially stimulates anti-U1A autoantibody production by splenic B cells from pristane-treated mice. a Purified (magnetic beads) splenic CD19⁺ B from pristane-treated mice were stimulated with toll-like receptor (TLR) agonists (R848, LPS, or CpG1826) for 10 days. IgG anti-U1A autoantibodies were measured in culture supernatants (ELISA). b Flow-sorted plasma cells (PC), switched plasmablasts (PB), switched memory B cells (MB), and naïve B cells (NB) were stimulated with PBS or R848 for 10 days, and IgG anti-U1A was measured (ELISA). c Flow-sorted splenic sMB cells were stimulated with TLR ligands for 10 days, and total IgG and IgG anti-U1A levels were measured in culture supernatants (ELISA). Data are representative of three experiments. d BALB/c and BALB/c TLR7−/− mice were treated with pristane and serum IgG and IgM autoantibody levels were determined (ELISA) 30 weeks later (1:40 serum dilution): *p <0.05, **p <0.01; ***p <0.001 (paired Student t test)