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Table 2 Antibodies used for immunohistochemistry

From: Nerves are more abundant than blood vessels in the degenerate human intervertebral disc

Target antibody Clonality Optimal dilution Antigen retrieval method Secondary antibody Serum block
NF200 (ab82259) Mouse monoclonal 1:400 Heat Rabbit anti-mouse Rabbit
PGP9.5 (ab8189) Mouse monoclonal 1:200 Heat Rabbit anti-mouse Rabbit
CD31 (ab28364) Rabbit polyclonal 1:400 Enzyme Goat anti-rabbit Goat
  1. CD31 cluster of differentiation factor 31, NF200 neurofilament 200, PGP protein gene product