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Table 2 Antibodies used for immunohistochemistry

From: Nerves are more abundant than blood vessels in the degenerate human intervertebral disc

Target antibody

Clonality

Optimal dilution

Antigen retrieval method

Secondary antibody

Serum block

NF200 (ab82259)

Mouse monoclonal

1:400

Heat

Rabbit anti-mouse

Rabbit

PGP9.5 (ab8189)

Mouse monoclonal

1:200

Heat

Rabbit anti-mouse

Rabbit

CD31 (ab28364)

Rabbit polyclonal

1:400

Enzyme

Goat anti-rabbit

Goat

  1. CD31 cluster of differentiation factor 31, NF200 neurofilament 200, PGP protein gene product