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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Hedgehog signalling does not stimulate cartilage catabolism and is inhibited by Interleukin-1β

Fig. 3

Hedgehog pathway activation has no effect on a disintegrin and matrix metalloproteinase with thrombospondin motif-5 (ADAMTS-5) and matrix metalloproteinase-13 (MMP-13) gene expression or on sulphated glycosaminoglycans (sGAG) release in human articular cartilage. Changes in the gene expression of ADAMTS-5 and MMP-13 in isolated human articular chondrocytes are shown after 24 hours (a) and 72 hours (b) of stimulation with recombinant Indian hedgehog (r-Ihh) compared to controls. Data are mean fold-change (n = 12 from three separate donors). c sGAG release for untreated controls, r-Ihh, and cyclopamine at 24-hour intervals over the 72-hour culture period, and cumulatively over the 72-hour culture period. Data are mean sGAG release, normalised to the untreated controls from the same donor tissue at the same time point, with standard deviation error bars (n = 6). *Statistically significant difference between groups and their respective untreated controls, *P <0.05, **P ≤0.01, and ***P ≤0.001

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