Fig. 4
From: Hedgehog signalling does not stimulate cartilage catabolism and is inhibited by Interleukin-1β
Hedgehog pathway modulation does not affect cartilage degradation in IL-1β-treated explants. a Changes in cumulative sulphated glycosaminoglycans (sGAG) release following treatment with IL-1β, from 0 to 24 hours, in bovine articular cartilage explants are shown over the 72-hour culture period. b The mean cumulative sGAG release at 72 hours for explants treated with IL-1β in the presence or absence of recombinant Indian hedgehog (r-Ihh) is displayed, compared to untreated controls. c Mean cumulative sGAG release at 72 hours for explants treated with IL-1β in the presence or absence of cyclopamine, compared to untreated controls. All data are mean sGAG release, normalised to untreated controls from the same animal at the same time point, with standard deviation error bars (n = 9 from three separate donors). *Statistically significant difference between the treatment groups and their respective untreated control, *P <0.05, **P ≤0.01, and ***P ≤0.001