Fig. 4

TNFα influences RasGRP1 and RasGRP3 gene expression levels in T and B cells respectively via TNFR1 and TNFR2. a Quantitative PCR analysis was performed to measure RasGRP1 and RasGRP3 gene expression levels in T and B cells, respectively, obtained from three buffy coats. In each condition, cells were cultured with or without TNFα for 24 and 48 hours. b TNF receptor (TNFR)1 and TNFR2 expression in B (CD19) and T cells (CD3) was checked by flow cytometry. c Quantitative PCR analysis was performed to measure RasGRP1 and RasGRP3 gene expression levels in T and B cells, respectively, from three buffy coats. In each condition, T or B cells, previously exposed to anti-TNFR1 or anti-TNFR2 neutralizing antibodies, were cultured with TNFα for 48 hours. The relative expression levels (in arbitrary units (AU)) of RasGRP1 and RasGRP3 were normalized with 18S RNA abundance. Mean ± standard error of the mean were compared using one-way analysis of variance followed by Bonferroni post-hoc test: *p <0.05; **p <0.01; ***p <0.001