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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Dysregulation of RasGRP1 in rheumatoid arthritis and modulation of RasGRP3 as a biomarker of TNFα inhibitors

Fig. 5

TNFα influences RasGRP1 and RasGRP3 protein expression levels respectively in T and B cells via TNF receptor (TNFR)1 and TNFR2. Western blot analysis was performed to measure RasGRP1 protein expression in T cells from five healthy controls (HC) (a) and RasGRP3 protein expression in B cells from three HC (b). In the control condition, cells were cultured without TNFα treatment. Cells were cultured with TNFα for 48 hours and with or without antibodies targeting TNFR1 or TNFR2. A value of 1 was arbitrarily assigned to control conditions (without TNFα treatment) to which glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was reported and expressed as fold change. Mean ± standard error of the mean were compared using one-way analysis of variance followed by the Dunnett post-hoc test: *p <0.05; **p <0.01

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