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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Osteogenic differentiation of bone marrow stromal cells is hindered by the presence of intervertebral disc cells

Fig. 3

Expression of bone morphogenic protein antagonists Chordin, Gremlin, and Noggin in intervertebral disc (IVD) cells. a Immunocytochemistry of GREM1 and NOG in monolayers of passage 1 nucleus pulposus cells (NPCs) (a), annulus fibrosus cells (AFCs) (b) and (c) mixed human IVD cells. GREM1 and NOG were both expressed in NPCs, whereas minimal expression of NOG, but not GREM1, was found in AFCs. GREM1 and NOG were highly expressed in mixed cultures of NPCs and AFCs. d Quantification of BMP antagonist gene expression in NPCs relative to AFCs (n = 8 donors) at passage 1. NPC monolayer culture showed no differences in CHRD or NOG expression compared with NPCs and AFCs. GREM1 expression was 2-fold higher in NPCs than in AFCs. DAPI 4',6-diamidino-2-phenylindole

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Arthritis Research & Therapy

ISSN: 1478-6362

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