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Table 3 Primer sequences for real-time PCR analyses

From: Osteogenic differentiation of bone marrow stromal cells is hindered by the presence of intervertebral disc cells

Gene Forward primer Reverse primer
18S CGA TGC GGC GGC GTT ATT C TCT GTC AAT CCT GTC CGT GTC C
Alkaline phosphatase (ALP) GTA TGA GAG TGA CGA GAA AAT AGG TAG TCC ACA TTG T
Runt-related transcription factor 2 (RUNX2) AGC AGC ACT CCA TAT CTC T TTC CAT CAG CGT CAA CAC
Osteopontin (OPN) ACG CCG ACC AAG GAA AAC TC GTC CAT AAA CCA CAC TATC ACC TCG
Noggin (NOG) CAG CAC TAT CTC CAC ATC CG CAG CAG CGT CTC GTT CAG
Gremlin (GREM1) GAG AAG ACG ACG AGA GTA AGG AA CCA ACC AGT AGC AGA TGA ACA G
Chordin (CHRD) GTG GCT GCT GTG ACT CTG AAC AGG ACA CTG CCA TTG