Table 3 Primer sequences for real-time PCR analyses
Gene | Forward primer | Reverse primer |
|---|---|---|
18S | CGA TGC GGC GGC GTT ATT C | TCT GTC AAT CCT GTC CGT GTC C |
Alkaline phosphatase (ALP) | GTA TGA GAG TGA CGA GAA | AAT AGG TAG TCC ACA TTG T |
Runt-related transcription factor 2 (RUNX2) | AGC AGC ACT CCA TAT CTC T | TTC CAT CAG CGT CAA CAC |
Osteopontin (OPN) | ACG CCG ACC AAG GAA AAC TC | GTC CAT AAA CCA CAC TATC ACC TCG |
Noggin (NOG) | CAG CAC TAT CTC CAC ATC CG | CAG CAG CGT CTC GTT CAG |
Gremlin (GREM1) | GAG AAG ACG ACG AGA GTA AGG AA | CCA ACC AGT AGC AGA TGA ACA G |
Chordin (CHRD) | GTG GCT GCT GTG ACT CTG | AAC AGG ACA CTG CCA TTG |