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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Soluble biglycan: a potential mediator of cartilage degradation in osteoarthritis

Fig. 1

Effect of soluble biglycan (sBGN) on the expression of proteinases associated with matrix remodelling and cartilage degeneration in osteoarthritis (OA). a In primary chondrocytes, sBGN increased all proteinases examined. Lipopolysaccharide (LPS) was used as a positive control. b In cartilage explants, sBGN increased ADAMTS5, MMP13, CTSK and MMP9 messenger RNA (mRNA) only in mild grade I OA and not in severe grade IV OA. The results are expressed as fold changes (mean ± SD) relative to TATA box-binding protein (TBP) housekeeper and compared with non-stimulated controls. Samples were run as technical duplicates, and each experiment was done using samples obtained from at least six different donors (biological replicates). *p < 0.05, **p < 0.01, ***p < 0.001 vs. non-stimulated controls; ## p < 0.01 for pairwise comparisons between mild grade I OA and severe grade IV OA. ADAMTS a disintegrin and metalloproteinase with thrombospondin type 1 motif (aggrecanase), MMP matrix metalloproteinase, CTSK cathepsin K

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