Table 1 Primer pairs used for real-time polymerase chain reactions

ADAMTS4

A disintegrin and metalloproteinase with thrombospondin motifs 4

Forward: 5′-AAT CCT GTC AGC TTG GTG GT-3′

Reverse: 3′-CTT GGA GTT GTC ATG GAG CA-5′

ADAMTS5

A disintegrin and metalloproteinase with thrombospondin motifs 5

Forward: 5′-CTT CAC TGT GGC TCA CGA AA-3′

Reverse: 3′-AAT GTC AGG TTG CAC TGC TG-5′

MMP13

Matrix metalloproteinase 13 (MMP-13)

Forward: 5′-CTA TGG TCC AGG AGA TGA AG-3′

Reverse: 3′-AGA GTC TTG CCT GTA TCC TC-5′

CTSK

Cathepsin K

Forward: 5′-ACC CAA CAG GCA AGG CAG CTAA-3′

Reverse: 3′-GCA ATG CCA CAGG CGT TGT TCT-3′

MMP9

Matrix metalloproteinase 9 (MMP-9)

Forward: 5′-TTC TCC AGA AGC AAC TGT CC-3′

Reverse: 3′-CGG CAA GTC TTC CGA GTA GT-5′

ACAN

Aggrecan

Forward: 5′-TGG TGA TGA TCT GGC ACGA-3′

Reverse: 3′-TCT GCG TTT GTA GGT GGTG-5′

COL2A1

Collagen, type II, alpha 1

Forward: 5′-GAG TCA AGG GTG ATC GTG GT-3′

Forward: 5′-AAG CAC CTT GGT CTC CAG AA-5′

TBP

TATA box-binding protein

Forward: 5′-GAA GAA CAA TCC AGA CTA GCA GCA-3′

Reverse: 3′-CCT TAT AGG GAA CTT CAC ATC ACAG-5′

GAPDH

Glyceraldehyde 3-phosphate dehydrogenase

Forward: 5′-AAG GTC ATC CCT GAG CTG AA-3′

Reverse: 3′-TGC TGT AGC CAA ATT CGT TG-5′