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Table 1 Primer pairs used for real-time polymerase chain reactions

From: Soluble biglycan: a potential mediator of cartilage degradation in osteoarthritis

Gene Protein name Primer sequence
ADAMTS4 A disintegrin and metalloproteinase with thrombospondin motifs 4 Forward: 5′-AAT CCT GTC AGC TTG GTG GT-3′
Reverse: 3′-CTT GGA GTT GTC ATG GAG CA-5′
ADAMTS5 A disintegrin and metalloproteinase with thrombospondin motifs 5 Forward: 5′-CTT CAC TGT GGC TCA CGA AA-3′
Reverse: 3′-AAT GTC AGG TTG CAC TGC TG-5′
MMP13 Matrix metalloproteinase 13 (MMP-13) Forward: 5′-CTA TGG TCC AGG AGA TGA AG-3′
Reverse: 3′-AGA GTC TTG CCT GTA TCC TC-5′
CTSK Cathepsin K Forward: 5′-ACC CAA CAG GCA AGG CAG CTAA-3′
Reverse: 3′-GCA ATG CCA CAGG CGT TGT TCT-3′
MMP9 Matrix metalloproteinase 9 (MMP-9) Forward: 5′-TTC TCC AGA AGC AAC TGT CC-3′
Reverse: 3′-CGG CAA GTC TTC CGA GTA GT-5′
ACAN Aggrecan Forward: 5′-TGG TGA TGA TCT GGC ACGA-3′
Reverse: 3′-TCT GCG TTT GTA GGT GGTG-5′
COL2A1 Collagen, type II, alpha 1 Forward: 5′-GAG TCA AGG GTG ATC GTG GT-3′
Forward: 5′-AAG CAC CTT GGT CTC CAG AA-5′
TBP TATA box-binding protein Forward: 5′-GAA GAA CAA TCC AGA CTA GCA GCA-3′
Reverse: 3′-CCT TAT AGG GAA CTT CAC ATC ACAG-5′
GAPDH Glyceraldehyde 3-phosphate dehydrogenase Forward: 5′-AAG GTC ATC CCT GAG CTG AA-3′
Reverse: 3′-TGC TGT AGC CAA ATT CGT TG-5′