Environmental cold exposure increases blood flow and affects pain sensitivity in the knee joints of CFA-induced arthritic mice in a TRPA1-dependent manner

Table 1 mRNA expression in arthritic mice exposed to cold

Gene	Tissue	RT		10 °C
		Saline	CFA	Saline	CFA
TRPA1	DRG	862.76 ± 62.84	1116.79 ± 109.20	1005.35 ± 98.58	879.87 ± 63.95
TRPA1	Synovial membrane	24.17 ± 5.48	20.09 ± 5.41	31.98 ± 6.98	7.69 ± 0.98^*
TRPA1	Patellar cartilage	41.20 ± 8.21	48.94 ± 10.15	45.56 ± 8.14	115.00 ± 42.37^*#
TAC-1	DRG	8939.63 ± 1547.03	10197.42 ± 911.50	11325.64 ± 1143.52	7620.78 ± 1708.66
TAC-1	Synovial membrane	346.28 ± 70.11	132.70 ± 16.78^*	362.31 ± 72.70	249.36 ± 99.34
TAC-1	Patellar cartilage	145.37 ± 42.49	86.91 ± 25.93	89.69 ± 24.76	214.74 ± 71.01
CGRP	DRG	309931.4 ± 38430.2	370243.6 ± 45187.4	391659.9 ± 35192.5	252244.4 ± 52594.3
CGRP	Synovial membrane	68.73 ± 15.55	48.38 ± 18.91	57.44 ± 6.89	24.98 ± 1.42
CGRP	Patellar cartilage	43.38 ± 8.47	32.64 ± 9.02	33.98 ± 7.48	120.63 ± 46.56^*#

TRPA1, TAC-1 and CGRP mRNA expression in DRG, synovial membrane, and patellar cartilage samples obtained from RT-and cold-exposed mice with 2-week CFA-induced monoarthritis. Data are represented as copy numbers per μl of pure cDNA normalized by comparison to B₂M, HPRT and GAPDH in contralateral (saline-injected) and ipsilateral (CFA-injected) sides, n = 4–17, ^* p < 0.05, relative to saline-treated counterparts, ^# p < 0.05 relative to RT-exposed CFA-treated counterparts
RT room temperature, CFA complete Freund’s adjuvant, DRG dorsal root ganglia

ISSN: 1478-6362