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Fig. 6 | Arthritis Research & Therapy

Fig. 6

From: The CII-specific autoimmune T-cell response develops in the presence of FTY720 but is regulated by enhanced Treg cells that inhibit the development of autoimmune arthritis

Fig. 6

The ratio of regulatory T (Treg) cells to helper T cells is increased in lymph nodes of FTY720-treated mice. Lymph node cells from DR1 transgenic mice immunized with type II collagen and treated with FTY720 or vehicle three times per week (total of eight doses) were recovered on day 17 and stained with antibodies specific for CD4, CD8, CD19, CD3, CD25, and Foxp3, and they were analyzed by flow cytometry. a and b Representative scatterplots (1 mg/kg FTY720 and vehicle control, respectively) of data from at least three repeat experiments, summarized in (c). Open bars indicate 1.0 mg/kg FTY720, and closed bars represent 1.0 mg/kg vehicle control. The number of Treg cells per lymph node shown in (d) was determined using CountBright beads as described in the Methods section. The differences observed were not statistically significant. The ratio of Treg cells to CD4+ cells shown in (e) was calculated by dividing the number of both cell populations measured using the CountBright beads. Flow cytometry data shown were derived from sequential gating on live cells (4′,6-diamidino-2-phenylindole–negative), forward versus side scatter, CD8− and CD19−, CD3+, and CD4+ cells. Error bars indicate standard deviation

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