Fig. 5

Complement-dependent lysis of β60-74Cit peptide-specific B cells induced by bifunctional PLGA NPs in the presence of NHS as measured by flow cytometry. a Upper panel: representative figure of a healthy donor, lower panel: typical result with an RA patient. The binding of fluorescein-loaded NPs was detected in channel 1, dead cells were detected after TO-PRO staining in channel 4. The β60-74Cit-positive, killed B cells are detected in the right upper quadrant of the dot plot. b Individual data of five RA patients. ELISA ratios of sera of the same RA patients were between 5 and 19. Data were analyzed with paired t test, means ± SD are shown (**p <0.01). iNHS heat-inactivated normal human sera, NHS normal human sera, PLGA poly(d,l-lactic-co-glycolic acid)